I-11: Optimal Strategy Toward Fertility Preservation

Background There are several indications of human female gamete cryostorage including sub-fertile and fertile patients. But our focus will be in women at risk of losing their reproductive function caused by oncologycal treatments or premature ovarian failure that could benefit greatly from this practice. Fertile women may take advantage of this technology to electively delay childbearing. The overall outcome of the oocyte using vitrified oocytes in fertility preservation is satisfactory although clinical experience is still limited. Survival rate after warming is over 90%. The potential of vitrified oocytes is consistently similar than the one attained for fresh ones. All the evidences described in this lecture indicate that nowadays oocyte and embryo cryopreservation by means of vitrification is providing a highly effective tool within ART, attaining similar outcomes than those obtained with fresh oocytesembryos, thus allowing its application into clinical practice. Special attention will be done also in sperm freezing-thawing process on Vitrification. This method for cryopreservation, without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen is an optimal protocol for sperm crypreservation devoted to male fertility preservation. In this lecture we will discuss some basic and practical aspects of vitrification and show evidences on outcomes of sperm, oocytes and embryo vitrification and its contribution to the improvement of fertility preservation. MaterialsAndMethods N;Results N;Conclusion N;